Regulatory
PhslVU

Part:BBa_K3773011:Design

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


hslVU promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was successfully used in a fusion with a lacZ reporter2; expression levels from the promoter were quantifiable.

Source

We obtained the promoter sequence of bases from Chuang et al., 19931, while the beginning of the promoter sequence was derived from the primer used by Lien et al., 20092.

References

1 Chuang, S. E., Burland, V., Plunkett III, G., Daniels, D. L., & Blattner, F. R. (1993). Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli. Gene, 134(1), 1-6.


2 Lien, H. Y., Yu, C. H., Liou, C. M., & Wu, W. F. (2009). Regulation of clpQ+ Y+ (hslV+ U+) gene expression in Escherichia coli. The Open Microbiology Journal, 3, 29.